Abstract
A hollow-fiber enzyme reactor, operating under isothermal and nonisothermal
conditions, was built employing a polypropylene hollow fiber onto which â-galactosidase
was immobilized. Hexamethylenediamine and glutaraldehyde were used as spacer
and coupling agent, respectively. Glucose production was studied as a function of
temperature, substrate concentration, and size of the transmembrane temperature
gradient. The actual average temperature differences across the polypropylene fiber,
to which reference was done to evaluate the effect of the nonisothermal conditions,
were calculated by means of a mathematical approach, which made it possible to know,
using computer simulation, the radial and axial temperature profiles inside the
bioreactor and across the membrane. Percent activity increases, proportional to the
size of the temperature gradients, were found when the enzyme activities under
nonisothermal conditions were compared to those measured under comparable
isothermal conditions. Percent reductions of the production times, proportional to the
applied temperature gradients, were also calculated. The advantage of employing
nonisothermal bioreactors in biotechnological industrial process was discussed.
Anno
2004
Autori IAC
Tipo pubblicazione
Altri Autori
Diano N.; Grano V.; Rossi S.; Bencivenga U.; Portaccio M.; Amato U.; Carfora F.; Lepore M.; Gaeta F.S.; Mita D.G.
Editore
Wiley
Rivista
Biotechnology progress (Print)