Dynamics and rheology of cells and vesicles in shear flow

A deep understanding of the dynamics and rheology of suspensions of vesicles, cells, and capsules is relevant for different applications, ranging from soft glasses to blood flow [1]. I will present the study of suspensions of fluid vesicles by a combination of molecular dynamics and mesoscale hydrodynamics simulations (multi-particle collision dynamics) in two dimensions [2], pointing out the big potential of the numerical method to address problems in soft matter.

A macroscopic mathematical model for cell migration assays using a real-time cell analysis

Experiments of cell migration and chemotaxis assays have been classically performed in the so-called Boyden Chambers. A recent technology, xCELLigence Real Time Cell Analysis, is now allowing to monitor the cell migration in real time. This technology measures impedance changes caused by the gradual increase of electrode surface occupation by cells during the course of time and provide a Cell Index which is proportional to cellular morphology, spreading, ruffling and adhesion quality as well as cell number.

Overproduction of indole-3-acetic acid in free-living rhizobia induces transcriptional changes resembling those occurring in nodule bacteroids.

Free-living bacteria grown under aerobic conditions were used to investigate, by next-generation RNA sequencing analysis, the transcriptional profiles of Sinorhizobium meliloti wild-type 1021 and its derivative, RD64, overproducing the main auxin indole-3-acetic acid (IAA). Among the upregulated genes in RD64 cells, we detected the main nitrogen-fixation regulator fixJ, the two intermediate regulators fixK and nifA, and several other genes known to be FixJ targets.

On the genome base composition of teleosts: the effect of environment and lifestyle

Background: The DNA base composition is well known to be highly variable among organisms. Bio-physic studies on the effect of the GC increments on the DNA structure have shown that GC-richer DNA sequences are more bendable. The result was the keystone of the hypothesis proposing the metabolic rate as the major force driving the GC content variability, since an increased resistance to the torsion stress is mainly required during the transcription process to avoid DNA breakage.

Ultrasensitive HCV RNA Quantification in Antiviral Triple Therapy: New Insight on Viral Clearance Dynamics and Treatment Outcome Predictors.

Objectives Identifying the predictive factors of Sustained Virological Response (SVR) represents an important challenge in new interferon-based DAA therapies. Here, we analyzed the kinetics of antiviral response associated with a triple drug regimen, and the association between negative residual viral load at different time points during treatment. Methods Twenty-three HCV genotype 1 (GT 1a n = 11; GT1b n = 12) infected patients were included in the study.

Numerical validation of the conjecture of a subglacial lake at Amundsenisen, Svalbard

The likelihood of a subglacial lake beneath Amundsenisen Plateau at Southern Spitzbergen, Svalbard, pointed out by the flat signal within the Ground Penetrating Radar (GPR) remote survey of the area, is justified, here, via numerical simulation.This investigation has been developed under the assumption that the icefield thickness does not change on average, as it is confirmed by recently published physical measurements taken over the past 40 years.

Experimental study on the atmospheric delay based on GPS, SAR interferometry, and numerical weather model data

In this paper, we present the results of an experiment aiming to compare measurements of atmospheric delay by synthetic aperture radar (SAR) interferometry and GPS techniques to estimates by numerical weather prediction. Maps of the differential atmospheric delay are generated by processing a set of interferometric SAR images acquired by the ENVISAT-ASAR mission over the Lisbon region from April to November 2009. GPS measurements of the wet zenith delay are carried out over the same area, covering the time interval between the first and the last SAR acquisition.

Clustering of vertically constrained passive particles in homogeneous isotropic turbulence

We analyze the dynamics of small particles vertically confined, by means of a linear restoring force, to move within a horizontal fluid slab in a three-dimensional (3D) homogeneous isotropic turbulent velocity field. The model that we introduce and study is possibly the simplest description for the dynamics of small aquatic organisms that, due to swimming, active regulation of their buoyancy, or any other mechanism, maintain themselves in a shallow horizontal layer below the free surface of oceans or lakes.

Prediction of energy losses in water impacts using incompressible and weakly compressible models

In the present work the simulation of water impacts is discussed. The investigation is mainly focused on the energy dissipation involved in liquid impacts in both the frameworks of the weakly compressible and incompressible models. A detailed analysis is performed using a weakly compressible Smoothed Particle Hydrodynamics (SPH) solver and the results are compared with the solutions computed by an incompressible mesh-based Level-Set Finite Volume Method (LS-FVM). Impacts are numerically studied using single-phase models through prototypical problems in 1D and 2D frameworks.